Analysis Note

ControlPOSITIVE CONTROL: Cytotrophoblastic columns, the endothelial and fibroblastic stromal cells of villi, and the large decidualized cells of decidual membrane in placenta1.

Application

Western Blotting: 1-2 µg/mL for 2 hours at room temperature

Immunohistochemistry: Formalin-fixed, paraffin-embedded tissues: 1-2 µg/mL for 30 min. at room temperature.

Optimal working dilutions must be determined by end user.

Research CategoryCell Structure

Research Sub CategoryMMPs & TIMPs

Anti-TIMP-1 Antibody, clone 102D1 is an antibody against TIMP-1 for use in WB, IH(P).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Recombinant human TIMP-1.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Purified from ascites fluid by Protein G chromatography. Liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Specificity

It recognizes a glycoprotein of 28.5 kDa which is identified as tissue inhibitor of metalloproteinases-1 (TIMP-1). TIMP-1 and TIMP-2 have similar properties, specifically in inhibiting enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-1 forms a complex of 1:1 stoichiometry with activated interstitial collagenase, activated stromelysin, active form of 72 kDa type IV collagenase (also known as MMP-2 or gelatinase A), and latent and active forms 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMPs inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Cellular Localization:

Cytoplasmic

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 12 months after date of receipt.